Association of ApoE gene polymorphism with coronary heart disease and lipid metabolism

Objective To investigate and the association of ApoE gene polymorphism with coronary heart disease(CHD).Methods The polymerase chain reaction restriction fragment length polymorphism technology(PCR-RFLP)was used to detect ApoE genotype in 88 CHD patients(according to WHO 1979 criterion) and 75 normal controls.TC、LDL-C、HDL-C、TG、ApoA1、ApoB-100 of fasting serum were measured by anto-biochemical analysis system.Results From our experiment,5 genotypes were found in both CHD and control groups: E2/3,E3/3,E3/4,E2/4 and E4/4.the frequence of E3/3 was highest in both groups.There was a significant difference of genotype distribution between CHD and control group,the frequence of E2/4、E4/4 in CHD group was higher than control,the frequence of E3/3 were lower than control.the frequence of e2 ande4 in CHD group was higher than control,and the frequence of e3 was lower than control.Conclusion Apolipoprotein E gene polymorphism affects serum TC,LDL-C and LP(a) levels in CHD.There was significant association of polymorphism of ApoE gene with CHD,while the distribution of the ApoE genotype was different among three groups.e4 allele is a risk factor in CHD.