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2015•Acta Academiae Medicinae Qingdao UniversitatisRequires access

ESTABLISHMENT OF LUCIFERASE EXPRESSION RECOMBINANT CONTAINING HUMAN GLUT9 GENE PROMOTER

Zhu Xuelia

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Abstract

Objective To establish the luciferase expression recombinant containing different haplotypes DNA(GG/CC)on SNP rs13124007 of human GLUT9 gene promoter. Methods Specific DNA fragments were amplified by PCR,and after being digested by restriction endonucleases,the fragments were then inverted into the digested pGL3-basic vector.The products were verified by endonuclease and sequencing. Results The verification indicated the recombinant contained both the sequence of pGL3-basic and rs13124007 of human GLUT9 gene promoter.The segment was inserted in the right direction. Conclusion The dual luciferase expression vector is successfully constructed,which lays a foundation for subsequent research on whether SNP rs13124007 affects the activity of promoter.

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What this paper is about

Objective To establish the luciferase expression recombinant containing different haplotypes DNA(GG/CC)on SNP rs13124007 of human GLUT9 gene promoter. Methods Specific DNA fragments were amplified by PCR,and after being digested by restriction endonucleases,the fragments were then inverted into the digested pGL3-basic vector.The products were verified by endonuclease and sequencing. Results The verification indicated the recombinant contained both the sequence of pGL3-basic and rs13124007 of human GLUT9 gene promoter.The segment was inserted in the right direction. Conclusion The dual luciferase expression vector is successfully constructed,which lays a foundation for subsequent research on whether SNP rs13124007 affects the activity of promoter.

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Available abstract

Objective To establish the luciferase expression recombinant containing different haplotypes DNA(GG/CC)on SNP rs13124007 of human GLUT9 gene promoter. Methods Specific DNA fragments were amplified by PCR,and after being digested by restriction endonucleases,the fragments were then inverted into the digested pGL3-basic vector.The products were verified by endonuclease and sequencing. Results The verification indicated the recombinant contained both the sequence of pGL3-basic and rs13124007 of human GLUT9 gene promoter.The segment was inserted in the right direction. Conclusion The dual luciferase expression vector is successfully constructed,which lays a foundation for subsequent research on whether SNP rs13124007 affects the activity of promoter.

Key concepts: Luciferase, Recombinant DNA, Restriction enzyme, Molecular biology, Promoter, Gene, Biology, DNA

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