Effects of bar-gene on microbial flora in wheat rhizosphere soil

Transgenic bar-gene wheat 87158(BG 87) and 98005(BG 98) are planted at pot as experiment material as well their parent wheat Yangmai 87158(YM 87) and Annong 98005(AN 98) as control.Effects of bar-gene on rhizosphere microbes of wheat were studied through analyzing the change of microbial flora in reizosphere soil at different wheat growth stages.The results indicated that the microbes′ quantity in rhizosphere soil is varied at different bearing stages of wheat.At seedling and tillering stage there is less microbes′quantity in rhizosphere soil,as well as at the booting and heading stage,the quantity is the most.Microbes′ quantity in rhizosphere soil decreased gradually from heading stage to filling stage of wheat growth.From elongation stage to late growth time,there is a significant difference on quantity of bacteria and fungi in rhizosphere soil between bar-gene wheat and control while there is no difference on quantity of actinomycete.bar-gene has no effects on microbes′ species in rhizosphere soil.