2006•Journal of Shandong UniversityRequires access

The role of bcl-2 and bax in the protection of hippocampal neurons by cerebral ischemic preconditioning against ischemia/reperfusion injury

Yajun Liu

Abstract

Objective: To investigate the role of apoptotic related genes,bcl2 and bax in the protection of hippocampal neurons by cerebral ischemic preconditioning(IPC) against ischemia/reperfusion injury in rats.Methods: Rats were randomly divided into ischemic preconditioning + ischemia reperfusion group(IPC), ischemia/reperfusion group(IR),and control group.Histological changes in the neurons of the hippocampal CA1 region were observed.The apoptosis of neurons in hippocampal CA1 area was determined by TUNEL staining.The expression of Bcl2 and Bax was detected with immunohistochemistry.Results: Cerebral ischemic preconditioning may increase the number of alive neurons in hippocampal CA1 region(P0.05),markedly reduce the apoptosis of pyramidal neurons(P0.05),and induce the expression of Bcl-2 and inhibit the expression of Bax(P0.05).Conclusion: Cerebral ischemic preconditioning(IPC) inducing upregulation of Bcl-2 expression and downregulation of Bax expression may inhibit the apoptosis and protect the hippocampal neurons against ischemia/reperfusion injury.

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Key concepts: Hippocampal formation, TUNEL assay, Ischemia, Downregulation and upregulation, Ischemic preconditioning, Apoptosis, Ischemic reperfusion injury, Neuroprotection