Expression of VEGF and PCNA and Microvessel Density in Osteosarcoma

Objective To study the role of the angiogenesis in osteosarcoma growth invasion and metastasis. Methods Immunohistochemical staining, using monoclonal antibodies was used to quantify the VEGF expression, the PCNA expression and the microvessel density(MVD)in 52 surgical specimens resected from patients with osteosarcoma. Anti-VEGF monochonal antibody was used to determine VEGF expression, anti-PCNA monoclonal antibody was used to determine PCNA expression. Results VEGF and MVD expression had significant correlation with metastasis ( P 0.05).VEGF and MVD expression had no correlation with tumor cell-type and tumor size ( P 0.05).PCNA expression had correlation with osteosarcoma metastasis ( P 0.05). Conclusion That angiogenesis is not only the basis of tumor of metastasis but also an important factor in tumor growth. PCNA expression could be the parameters in the judgment of postoperation metastasis and prognosis.