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2010•China Food AdditivesRequires access

Screen of high-yielding strains for D-lactic acid production

YU Pei-xing

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Abstract

Bacillus coagulans JD-063D was used as the starting strain,then mutagenized by UV,diethyl sulfate,cobalt 60 gamma radiation and microwave induction.Mutants were then picked up for fermentation detection and genetic stability test.A strain of JD-76D was selected.Its acid producing was increased from 91g /L to 145g /L,D-lactic acid purity was increased from 97.5% by the original strain to more than 98.7%.

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What this paper is about

Bacillus coagulans JD-063D was used as the starting strain,then mutagenized by UV,diethyl sulfate,cobalt 60 gamma radiation and microwave induction.Mutants were then picked up for fermentation detection and genetic stability test.A strain of JD-76D was selected.Its acid producing was increased from 91g /L to 145g /L,D-lactic acid purity was increased from 97.5% by the original strain to more than 98.7%.

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Available abstract

Bacillus coagulans JD-063D was used as the starting strain,then mutagenized by UV,diethyl sulfate,cobalt 60 gamma radiation and microwave induction.Mutants were then picked up for fermentation detection and genetic stability test.A strain of JD-76D was selected.Its acid producing was increased from 91g /L to 145g /L,D-lactic acid purity was increased from 97.5% by the original strain to more than 98.7%.

Key concepts: Bacillus coagulans, Lactic acid, Fermentation, Strain (injury), Food science, Mutant, Chemistry, Biochemistry

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