Effect of Bushen Fumai Liquid on eNOSmRNA and ET-1mRNA in Hypoxic Endothelial Cells

Objective: To observe the effect of Bushen Fumai Liquid (BSFML) on nitric oxide synthase endothelial type (eNOS) and endothelin mRNA (ET 1mRNA) in cultured human umbilical vein endothelial cells (HUVECs) induced by hypoxia. Methods: The cultured HUVECs were randomly divided into 4 groups, the blank group (A), the hypoxic group (B), the western medicine group (C) and the BSFML group (D). 10% blank rabbit serum was given to group A and B, 10% rabbit serum containing western medicine vit C and containing BSFML was given to Group C and D respectively. Except for Group A, the other 3 groups were exposed to hypoxia (95% N 2+5% CO 2) for 4 hours. The total RNA was extracted from the cultured HUVECs by quanidinium thiocynate method, then the reverse transcriptase polymerase chain reaction (RT PCR) was performed. The RT PCR products were fractionated through a 1.0% agarose gel electrophoresis, the eNOSmRNA and ET 1mRNA expression was observed under ultraviolet light and photographed, then bands on film were scanned by densitometer and quantified on computer by the image analysis software. Results: The highest ET 1mRNA level and lowest eNOSmRNA was shown in the hypoxic group, both abnormal expressions were inhibited in Group C and D (P0 01 or P0 05), and the inhibitory effect of BSFML was superior to that of western medicine (P0 05). Conclusion: BSFML could inhibit the ET 1mRNA transcription and promote eNOSmRNA transcription in hypoxic endothelial cells, so as to keep a balance between them. By this way, it could be effective in protecting hypoxic endothelial cells.