Effect of Xinmailing Injection on Lipopolysaccharide-induced Secretion of Endothelin-1 and Nitric Oxide from Endothelial Cells of Human Umbilical Veins

Objective To observe the effect of Xinmailing Injection (XI) on lipopolysaccharide(LPS)- induced secretion of endothelin- 1 (ET- 1) and nitric oxide (NO) from endothelial cells of human umbilical veins.Methods The in- vitro culture of human umbilical vein endothelial cells were stimulated by LPS( 100 μ g/L) and incubated with XI(320 g /L) for half an hour,one hour,2 hours,4 and 6 hours respectively.Then the cultured supernatants were collected.The viabilities of cells were measured by MTT method.The concentrations of ET- 1 and NO were determined by radioimmunoassay method and by Gneiss's Method respectively.Results (1) The viabilities of cells arrived the highest level as treated with XI with the concentration of 320 g /L (P 0.01 as compared with model group);(2) At each time point, the concentrations of ET- 1 (pg/L)of XI groups were significantly decreased (P 0.01 as compared with model group);the concentration of NO(μ mol/L) in XI groups were increased (P 0.01 as compared with model group at 2nd,4th and 6th hour).Conclusion XI can protect human umbilical vein endothelial cells from LPS- induced injury,and it can also regulate the secretion of ET- 1 and NO, which are the contracting and expanding cytokines of the blood vessels.