Comparison of Candida albicans Biofilms Model Systems in vitro

OBJECTIVE To compare the results of three kinds of Candida albicans biofilm model systems in vitro,and observe the dynamic course of C.albicans biofilm formation.METHODS C.albicans model strain ATCC 90038 formed biofilm structure on 96 well microtiter plates,on the glass plates in 6 well cell culture plates,and on the continuous culture systems-chemostat,which was observed by fluorescent microscope,and by XTT reduction method to quantitatively analyze the formation of biofilm.RESULTS All of the three kinds of biofilm model systems could form mature biofilm structure,and the quantitative analysis of biofilm formation indicated that three methods were significantly correlated.CONCLUSIONS These three model systems are all ideal methods for studying the C.albicans biofilm,we should choose the appropriate method according to objective of the study.