2011•Liaoning Zhongyiyao Daxue xuebaoRequires access

Comparative Study of Different Extraction Methods to Determine Chlorogenic Acid Content in YMZ

Fan Yong-chun

Abstract

Objective:To study the YMZ in the content determination of chlorogenic acid in the extraction methods and quality control of chlorogenic acid standards.Methods:High performance liquid chromatography to compare the different extraction methods,extraction solvent,extraction time and solvent consumption wild chase determination of chlorogenic acid content of herbs.Results:The use of ultrasonic extraction method to recover the herbs in chlorogenic acid,and chlorogenic acid by HPLC with sampling in the 0.0908~1.362μg(r = 0.9999)showed a good linear relationship between the average recovery was 100.12%,RSD 1.31%.Conclusion:The ultrasonic extraction of chlorogenic acid content was determined by high performance liquid method is simple and can be used for quality control of the medicine.

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Key concepts: Chlorogenic acid, Chromatography, Extraction (chemistry), High-performance liquid chromatography, Medicine, Content determination, Solvent, Chemistry