Extraction Technology and Content Determination of Chlorogenic Acid in Jinbanqing Granules

To establish the optimal extraction technology and to determine the content of effective constituents of chlorogenic acid in Jinbanqing granules.Nine experiments were carried out through L9(34) orthogonal design.The contents of chlorogenic acid were determined by HPLC.The optimal extraction process was as follows: 12 times amount water and extracted for 3 times,2.5 hours each time.Chlorogenic acid was linear within the range of 10.0～100 μg/mL,r=0.9999.The average recovery is 100.75%(RSD 1.62%,n= 6).The extraction process is scientific and the determination method is suitable for quality control of Jinbanqing granules.