Physical chemistry properties of nematode-trapping fungus-Arthrobotrys oligospora

Dyeing properties,resistance of conidia in Vitro to physical chemistry factors and attractive ability of hyphae to nematodes L3 were studied.The results showed that the fungus could be dyed well by the methods of Gram’s stain,Wright’s stain and Giemsa’s stain.The conidia had a good endurance under low frozen temperature and was not sensitive to antibiotics,but it was sensitive to high temperature.The H+ and OH- of high concentration could kill the conidia.the result indicated that conidia have a good ability of enduring under low frozen temperature.So we can use the freezing technique,a simple and easy method,to keep the actities of nematode-trapping fungi.Also,0.4g/L LCMA could be used to preserve the fungi isolates.The hyphae had not an obvious attractive ability to the third larvae of nematodes.