Novel approach to cellulose degradation with cellulase and acids

The influence of acids(HAc,HCl) on degrading cellulose of cellulase was studied with haulm.There are two schemes to be designed to decompose cellulose: Scheme one is enzyme-lyses after acids-lyses;Scheme two is acids-lyses after enzyme-lyses.A novel approach to depolymerize cellulose-cycling degrading with cellulase and acids of cellulose(CDCA) was researched based on the results of the former two schemes.The experimental results show that Scheme two is superior to Scheme one.The CDCA method is obviously superior to the former two schemes.The researched results of CDCA indicate that the transformation rate of cellulose to glucose(TR) and concentration of glucose in the end-residue can increase up to 98.68% and 5.38%,respectively with cellulase and CH3COOH,HCl at the temperature lower than 100 ℃,10 kPa,and for 12?15 h.It consumes 25% acetic acid and 50% HCl and a little enzyme compared with the traditional way.The technology of joint cellulase and acids(JCA) for decomposing cellulose requires mild condition,simple process,lower cost and shorter period.It can set a foundation for the development and production of ethanol,antibiotics and SCP with cellulose in the industry of fermentation and others such as solid waste bioprocessing.