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2010•China Animal Husbandry & Veterinary MedicineRequires access

Study on the Method of Improving Gene Transfection Efficiency

Bichun Li

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Abstract

The study aimed at improving gene transfection efficiency. We used PEI-mediated plasmid pEGFP-N1 to transfect NIH-3T3 cells in this study. NIH-3T3 Cells were divided into two groups to conduct one and two transfection,and observed under fluorescent inverted microscope at 48 h after transfection. The results showed that some cells emited green fluorescence,and green fluorescent cells in two transfection group were more than one transfection group. Calculated transfection efficiency to showed that two transfection method could significantly improve gene transfection efficiency.

About this research paper

What this paper is about

The study aimed at improving gene transfection efficiency. We used PEI-mediated plasmid pEGFP-N1 to transfect NIH-3T3 cells in this study. NIH-3T3 Cells were divided into two groups to conduct one and two transfection,and observed under fluorescent inverted microscope at 48 h after transfection. The results showed that some cells emited green fluorescence,and green fluorescent cells in two transfection group were more than one transfection group. Calculated transfection efficiency to showed that two transfection method could significantly improve gene transfection efficiency.

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Method / approach

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Available abstract

The study aimed at improving gene transfection efficiency. We used PEI-mediated plasmid pEGFP-N1 to transfect NIH-3T3 cells in this study. NIH-3T3 Cells were divided into two groups to conduct one and two transfection,and observed under fluorescent inverted microscope at 48 h after transfection. The results showed that some cells emited green fluorescence,and green fluorescent cells in two transfection group were more than one transfection group. Calculated transfection efficiency to showed that two transfection method could significantly improve gene transfection efficiency.

Key concepts: Transfection, Molecular biology, Fluorescence microscope, Green fluorescent protein, 3T3 cells, Plasmid, Fluorescence, Gene

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