Simultaneous Fluorospectrophotometric Determination of Norfloxacin and Levofloxacin in Plasma Using the Algorithm of 2nd Order Calibration

Simultaneous determination of norfloxacin and levofloxacin by fluorospectrophotometry with scanning in the excitation wavelength range of 265-510 ran and in the emission wavelength range of 300-650 run. Data of fluorescence intensity measured were treated by the algorithms of 2nd order calibration and 3-dimensional data array analysis. As analyzed by the algorithm of AATLD, values of average recovery of 96. 9% (for norfloxacin) and 103. 9% (for levofloxacin) were found. It was shown by the analytical results of the method proposed, the interferences due to overlapping of fluorescence spectra of the 2 analytes and also those unknown coexisting substances present in the sample, were effectively eliminated. The present method was proved to be simple and rapid for analysis of plasma samples without the necessity of pre-separation.