Study on the level of MMP-3 in synovium and synovial fluid in patitents with osteoarthritis

Objective To investigate the level of MMP-3 in synovium and synovial fluid of patitents with primary osteoarthritis (OA),and study the role of MMP-3 in OA.Methods The synovium and synovial fluid of knee joint were collected from 43 subjects with meniscus injury,45 adult patients with OA.The expression of MMP-3 in synovium was analyzed by immunohistochemistry staining.The level of MMP-3 was determined by ELISA.Results The intensity of positive cells with expression of MMP-3 in synovium was significantiy higher in patients OA than in subjects with meniscus injury (P0.01).The level of MMP-3 in patients with OA was significantiy higher than in subjects with meniscus injury in synovial fluid (P0.05).The level of MMP-3 in synovium and synovial fluid was not statistical significance in patients between one degree and two degree typed with X radioactive ray.Conclusion MMP-3 plays significant role in the pathogenesis of OA.