2010•Acta Agriculturae Boreali-SinicaRequires access

Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs

Sun Jun-she

Abstract

A full length cDNA library of Hoplobatrachus rugulosus skin tissue was constructed with the SMART technique.A special primer was designed from a highly conserved domain of the signal peptide of antimicrobial peptide encoding cDNAs from ranid frogs.Two different cDNA sequences encoding precursor proteins named tigerinin-HR1 and tigerinin-HR2 were screened from the library by PCR.tigerinin-HR1 and tigerinin-HR2 were the first found encoding cDNA sequences of antimicrobial peptide from H.rugulosus.

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Key concepts: Complementary DNA, cDNA library, Biology, Antimicrobial, Primer (cosmetics), Antimicrobial peptides, Signal peptide, clone (Java method)