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A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa

XU Biao

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Abstract

Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-β-lactamases,and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant,and the sensitivity rates of AMK,CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69),respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.

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Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-β-lactamases,and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant,and the sensitivity rates of AMK,CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69),respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.

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Available abstract

Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-β-lactamases,and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant,and the sensitivity rates of AMK,CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69),respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.

Key concepts: Imipenem, Pseudomonas aeruginosa, Microbiology, Drug resistance, Gene, Biology, Multiple drug resistance, Bacteria

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