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2011•Journal of China Pediatric Blood and CancerRequires access

Effects of allicin on cell growth and apoptosis in human leukemic Jurkat cell line

XU Zhi-lian

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Abstract

Objective To study the effects of allicin on cell growth and apoptosis in human leukemic Jurkat cell line. Methods Electrophoresis mobility shift assay (EMSA) was used to detect the DNA binding activity of NF-κB before and after allicin treatment. The cell growth and apoptosis in Jurkat cells were detected by MTT assay and flow cytometry (FCM) respectively. Results The DNA binding activity of NF-κB decreased after allicin treatment. The growth of Jurkat cells was suppressed in a dose-and time-dependent manner. Conclusion Cell growth inhibition by allicin is related to apoptosis in Jurkat cells, which may take effects through the NF-κB signal pathway.

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What this paper is about

Objective To study the effects of allicin on cell growth and apoptosis in human leukemic Jurkat cell line. Methods Electrophoresis mobility shift assay (EMSA) was used to detect the DNA binding activity of NF-κB before and after allicin treatment. The cell growth and apoptosis in Jurkat cells were detected by MTT assay and flow cytometry (FCM) respectively. Results The DNA binding activity of NF-κB decreased after allicin treatment. The growth of Jurkat cells was suppressed in a dose-and time-dependent manner. Conclusion Cell growth inhibition by allicin is related to apoptosis in Jurkat cells, which may take effects through the NF-κB signal pathway.

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Available abstract

Objective To study the effects of allicin on cell growth and apoptosis in human leukemic Jurkat cell line. Methods Electrophoresis mobility shift assay (EMSA) was used to detect the DNA binding activity of NF-κB before and after allicin treatment. The cell growth and apoptosis in Jurkat cells were detected by MTT assay and flow cytometry (FCM) respectively. Results The DNA binding activity of NF-κB decreased after allicin treatment. The growth of Jurkat cells was suppressed in a dose-and time-dependent manner. Conclusion Cell growth inhibition by allicin is related to apoptosis in Jurkat cells, which may take effects through the NF-κB signal pathway.

Key concepts: Jurkat cells, Allicin, Apoptosis, Cell growth, Flow cytometry, Cell culture, Molecular biology, Electrophoretic mobility shift assay

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