Study of Inhibiting The Expression of Survivin in Pancreatic Cancer Cells with RNA Interference

Objective To investigate the inhibitory effects of RNA interference(RNAi)expression vector on the expression of survivin in pancreatic cancer cell PANC-1.Methods The protein and mRNA expressions of survivin were examined with immunofluorescence and RT-PCR.The survivin gene was cloned into the T-vector and sequenced.The RNAi expression vectors targeting survivin,named si-svv-1 and si-svv-2 respectively according to whether they harbored a mutation or no mutation,were constructed and transfected into PANC-1 cells with liposome.The expression of survivin mRNA was detected with RT-PCR.Apoptosis of PANC-1 cells was analyzed with DNA ladder and FACS.Results There was a high degree expression of survivin in PANC-1 cells.The expression of survivin was not inhibited by RNAi expression vectors si-svv-1,but inhibited about(72.43±8.04)% by si-svv-2 and the apoptosis rate of PANC-1 cells increased to(12.36±1.44)% after 72 h.Conclusion The RNAi expression vector can effectively inhibit the expression of survivin in pancreatic cancer cell PANC-1 cells and induce the apoptosis in PANC-1 cells.