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2004•Journal of Hebei UniversityRequires access

Resonance Light Scattering Analysis of DNA by Using Cobaltic Hexammonate Ion as Probe

Zhao Rong-chao

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Abstract

In a Britton-Robinson (BR) buffer of pH 1.81～4.1, the interaction of [Co(NH_3)_6]~(3+) with DNA results in strong enhancement of resonance light scattering (RLS) intensity. Ander the optical condition, the enhanced RLS intensity at 342.0 nm was proportional to the concertration of DNA in the range of 0.01～(7.0 mg/L.) A sensitive and convenient new analysis method for microdetermination of DNA was accordingly established. The limit of dertermination was 1.6 μg/L (3σ) for Calf thymus DNA (ctDNA).

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What this paper is about

In a Britton-Robinson (BR) buffer of pH 1.81～4.1, the interaction of [Co(NH_3)_6]~(3+) with DNA results in strong enhancement of resonance light scattering (RLS) intensity. Ander the optical condition, the enhanced RLS intensity at 342.0 nm was proportional to the concertration of DNA in the range of 0.01～(7.0 mg/L.) A sensitive and convenient new analysis method for microdetermination of DNA was accordingly established. The limit of dertermination was 1.6 μg/L (3σ) for Calf thymus DNA (ctDNA).

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Available abstract

In a Britton-Robinson (BR) buffer of pH 1.81～4.1, the interaction of [Co(NH_3)_6]~(3+) with DNA results in strong enhancement of resonance light scattering (RLS) intensity. Ander the optical condition, the enhanced RLS intensity at 342.0 nm was proportional to the concertration of DNA in the range of 0.01～(7.0 mg/L.) A sensitive and convenient new analysis method for microdetermination of DNA was accordingly established. The limit of dertermination was 1.6 μg/L (3σ) for Calf thymus DNA (ctDNA).

Key concepts: Detection limit, DNA, Ion, Resonance (particle physics), Scattering, Intensity (physics), Light scattering, Chemistry

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