Expression and clinical significance of P-gp in acute lymphoblastic leukemia

Objective To detect the expression of P-glycoprotein(P-gp) in acute lymphoblasticleukemia(ALL)and evaluate the relationship between P-gp expression and prognosis of ALL.Methods The expression of anti-glycoprotein monoantibody:UIC2 and CD3,CD5,CD7,CD10,CD19,CD13,CD14,CD33,CD34 in 40 patients was defected with direct immunofluorescence assays by Flow Cytometry.The leukemia cells in peripheral blood and bone marrow should be more than fifty percent.The cell lineage of non-drug resistance,K_(562),was used as negative control.Results Sixteen patients in 40 expressed P-gp.P-gp positive rate in myeloid antigen positive cases was 33.3%.P-gp positive rate in myeloid antigen negative cases was 45.5%,P0.05.Four cases of T-cell acute lymphoblastic leukemia expressed CD7 and P-gp at the same time.Treatment was effective in ten of sixteen cases in P-gp positive group and effective in 20 of 24 cases in Pgp negative group(P(0.05)),while six cases in P-gp positive cases relapsed in six months.Conclusion P-gp positive rate in ALL is 40%.There is not relationship between expression of P-gp and myeloid antigens.The P-gp expression correlates with the CD7 expression.The P-gp expression correlates with chemotherapeutic effect in ALL.P-gp positive cases are prone to relapsing earlier.