The study on diagnostic value of the Immunogold-Dipstick reagent developed with Sj26-Sj32 recombinant protein for chronic schistosomiasis japonica.

Objective To evaluate the diagnostic value of the Immunogold-Dipstick reagent developed with Sj26-Sj32 recombinant protein for chronic schistosomiasis japonica.Methods The Immunogold-Dipstick method with Sj26-Sj32 recombinant protein and Schistosoma japonicum adult worms antigen(SjAWA) were used for detecting IgG in sera of chronic schistosomiasis japonica patients,sera from the patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,tuberculosis and healthy people were used as control.Results The sensitivity and specificity of this method were 92.50% and 97.67%,respectively.The positive predictive value,negative predictive value and diagnosis efficiency were 97.37%,93.33% and 95.18%,respectively.There was no cross reaction with sera of the mentioned parasitic diseases.Conclusion The recombinant Sj26-Sj32-Immunogold-Dipstick reagent may be used to immunodiagnosis of chronic schistosomiasis japonica.