Determination of Four Components in Forsythia suspensa from Different Habitats and Different Processed Products by HPLC

Objective:The HPLC method was developed to determine Caffeic acid,Forsythoside A,Forsythin,Arctigenin in Forsythia suspense from different habitats and different processed products.Method: Using RP-HPLC,Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm)columns,mobile phase acetonitrile-0.4% aqueous acetic acid solution by HPLC linear gradient elution method.The detection wavelength 0-20 min,323 nm,20-30 min,330 nm,30-50 min,277 nm,50-55 min,280 nm,column temperature 30 ℃,velocity 1.0 mL·min-1,the injection volume 20 μL.Result: The level of four components in F.suspensa from different habitats and different processed products is significantly different.Conclusion: The method can be used for the simultaneous determination of four components in F.suspensa,which provides a scientific basis for quality assessment to F.suspensa.