Establishment and indentification of mice model of radtion-induced lung injury

Objective To establish a mice model of radiation-induced lung injury and effective quantitative assessment methods.Methods Seventy-two Kunming female mice were assigned randomly to control group(recevied sham-irradiation,n=18)and treatment group(recevied irradition on the thoraces with single fraction 20-gray X-rays,n=54).The mice were sacrificed at 1,3 days and 1,2,4,7 weeks after the irradiation,lung tissues and venous blood were extracted to observe the pathological changes collagen fiber deposition of lung tissue by HE and Massion staining,and measure the content of TGF-β1 in blood serum via ELISA methods.Results Compared with the control group,significant effusion eg.vasocongestion,edema of lung interstitial substance and pulmonary capillary was observed in the treatment group at early stage after irradiation.At late stage after irradiation,cellulose effusion was main in alveolar septum and alveolar space.The difference of TGF-β1 content was significant(P0.01)between treatment group(18.46±1.678)ng/mL and control group(9.61±2.027)ng/mL at 7 days after irradiation by ELISA method.Conclusion A mice model of radition-induced lung injury that was confirmed by the pathological morphology and blood serum index was established succussfully.