2014•Unpublished venueRequires access
Effect of extraction of radix isatidis on the expression levels of TGF-β in lung tissues of rats with radioactive lung injury
Bingbing Zheng
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Abstract
Objective To investigate the effect of extraction of radix isatidis on the expression of transforming growth factor( TGF-β) in lung tissues of rats with radioactive lung injury. Methods 72 female Wistar rats were randomly divided into 3 groups,blank control group( groupA,n = 24),simple irradiation group( group B,n = 24),irradiation with Chinese medicine group( group C,n = 24). Rats in group A and group B were given distilled water2 mL /200 g by gavage for one week,the rats in group C were given extraction of radix isatidis 2 mL /200g( 1. 6 g /200 g) per day for one week,then the rats received irradiation,during irradiation,the rats' weight was measured once a week,to illuminate the end. After 10-time irradiation( the 30thday),and at 60 days and at 120 days after irradiation,the expression levels of TGF-β in lung tissue were detected; In addition each rat lung tissue morphology was observed. Results The expression levels of TGF-β protein in group B reached peak at the 30 days after irradiation,which at different time points in group A and group C was significantly lower than that in group B( P 0. 01). Rats of group A had no significant change in lung tissue. Rats of group B were observed inflammatory cells in interstitial pulmonary alveolar,alveolar structural damaging,interstitial pulmonary edema,interstitial hemorrhage and inflammatory pulmonary with alveolar cavity; Rats of group C pulmonary interstitial fewer inflammatory cells in alveolar damage were not obvious,with no-pulmonary interstitial edema, hemorrhage and no inflammatory symptoms, comparison between group B and C was not obvious.Conclusion Extraction of radix isatidis could reduce the expression levels of transforming growth factor( TGF-β) in lung tissues of rats with radioactive lung injury,has better preventive effect on radioactive lung injury,could protect lungtissue from damaging,inhibite alveolar permeability,and present the effect of pulmonary edema.