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Remittent effect of ischemic preconditioning on lung injury induced by ischemia-reperfusion in the hind limbs of rats

Jun Yang

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Abstract

Objective: To study the effect of ischemic preconditioning(IPC) on the lung injury following ischemia-reperfusion(I/R) in the hind limbs of rats. Methods: The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours. The tissue morphology, wet-to-dry weight(W/D) ratio, malondialdehyde (MDA), and myeloperoxidase (MPO) in lung were compared. The mRNA expression of ICAM-1 in lung were determined by using RT-PCR or in situ hybridization. The protein product was detected by Western blot. Results: In I/R group, all parameters increased significantly compared with sham operation or ischemia group (P0.01). In IPC+I/R group, the indices decreased significantly compared with I/R group (P0.01). Conclusion: The IPC can attenuate lung injury following I/R in the hind limbs of rats, which may be accomplished by inhibition of adhesion and infiltration of polymorphonuclear cell in lung after ICAM-1.

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Objective: To study the effect of ischemic preconditioning(IPC) on the lung injury following ischemia-reperfusion(I/R) in the hind limbs of rats. Methods: The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours. The tissue morphology, wet-to-dry weight(W/D) ratio, malondialdehyde (MDA), and myeloperoxidase (MPO) in lung were compared. The mRNA expression of ICAM-1 in lung were determined by using RT-PCR or in situ hybridization. The protein product was detected by Western blot. Results: In I/R group, all parameters increased significantly compared with sham operation or ischemia group (P0.01). In IPC+I/R group, the indices decreased significantly compared with I/R group (P0.01). Conclusion: The IPC can attenuate lung injury following I/R in the hind limbs of rats, which may be accomplished by inhibition of adhesion and infiltration of polymorphonuclear cell in lung after ICAM-1.

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Available abstract

Objective: To study the effect of ischemic preconditioning(IPC) on the lung injury following ischemia-reperfusion(I/R) in the hind limbs of rats. Methods: The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours. The tissue morphology, wet-to-dry weight(W/D) ratio, malondialdehyde (MDA), and myeloperoxidase (MPO) in lung were compared. The mRNA expression of ICAM-1 in lung were determined by using RT-PCR or in situ hybridization. The protein product was detected by Western blot. Results: In I/R group, all parameters increased significantly compared with sham operation or ischemia group (P0.01). In IPC+I/R group, the indices decreased significantly compared with I/R group (P0.01). Conclusion: The IPC can attenuate lung injury following I/R in the hind limbs of rats, which may be accomplished by inhibition of adhesion and infiltration of polymorphonuclear cell in lung after ICAM-1.

Key concepts: Malondialdehyde, Lung, Hindlimb, Medicine, Myeloperoxidase, Ischemia, Western blot, Reperfusion injury

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