Effect and mechanism of ischemic postconditioning on lung injury induced by ischemia-reperfusion of skeletal muscle in the hind limbs of rats

Objective:To study the effect of ischemic postconditioning(I-postC)on the lung injury following ischemia-reperfusion(I/R)of skeletal muscle in the hind limbs of rats.Methods:The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours.Forty-eight rats were divided into 3 groups:I/R group,IPC and I-postC group.Each group received 4 hours of ischemia and then 12 or 24 hours of reperfusion respectively.The tissue morphology,wet-to-dry weight(W/D)ratio,malondialdehyde(MDA)and myeloperoxidase(MPO) of lung tissue were compared.The expression of ICAM-1 mRNA in lung was also studied by RT-PCR or in situ hybridization.The protein product was detected by Western blot.Results:In IPC and I-postC groups,all parameters decreased significantly compared with I/R ischemia group(P0.01).There was no significant difference between the IPC and I-postC groups(P0.05).Conclusion:I-postC can attenuate lung injury following skeletal muscle I/R in the hind limbs of rats,which maybe accomplished by inhibition of adhesion and infiltration of PMN.