EXPERIMENTAL STUDY ON THE EFFECT OF PROTOPORPHYRIN DISODIUM IN VITRO ON ANTI-HBV

[Objective]To study the in-vitro inhibitation of Hepatitis B virus of NAPP and the cytotoxicity to HepG2.2.15 cell strain.[Methods]The cell toxicity of NAPP was determined with MTT method,and the effect of NAPP on HBsAg and HBeAg extracted by HepG2.2.15 cells was determined under diffenent concentrations on the ninth day with ELISA method.Real-time fluorescence quantitative PCR was used for determining extracted HBV-DNA in the culture medium.[Results]NAPP had no obvious cell toxicity at the concentration of 1mg/ml and depressed duplication of HBV DNA.NAPP had obvious restrain effect on HBsAg and HBeAg.The therapeutic index of NAPP was 188.7 for HBsAg and 64.5 for HBeAg.[Conclusion]NAPP has no obvious immediate cell toxicity on the cells and can inhibit the duplication of Hepatitis B Virus in Vitro.