Effects of soluble CD40L on hepatic injury of rats with obstructive jaundice

Objective This paper is to study the effect of soluble CD40L(sCD40L)on hepatic injury of rat with obstructive jaundice and its correlation with severity of liver damage.Methods Eighteen male SD rats were evenly and randomly divided into model of obstructive jaundice group,treatment of anti-CD40L monoclonal antibody(anti-CD40LmAb)group and control group.The rats of the model group were cut open the abdomen,dissociated the bile duct and double ligation,and closed the abdomen.After treating with the same method,the anti-CD40LmAb group was given anti-CD40LmAb intraperitoneal injection.The control group was only dissociated the bile duct and closed the abdomen.Three groups were detected of the serum levels of ALT,AST,TBIL,DBIL and TNF-α,IL-8,and sCD40L at different times.Results Compared with the model group,the ALT,AST,TNF-α,IL-8 and sCD40L levels of the anti-CD40LmAb group were significantly lower(P 0.05),but the TBIL and DBIL levels had no significant difference(P0.05).The serum sCD40L was positively correlated to those of TNF-αand IL-8.Conclusions sCD40L probably participate in the startup phase of hepatic injury of obstructive jaundice immune.Blocking the CD40/CD40L passway may be one of the effective methods for treatment of hepatic injury. sCD40 is expected to become a new indicator for hepatic damage degree.