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Cloning and sequencing of Tomato fruit sucrose phosphate synthase gene

Sun Li-ping

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Abstract

PCR primers were designed based on the consensus domain of some sucrose phosphate synthase genes registered in GenBank. A aimed complete cDNA was obtained by using RT-PCR from tomato fruit . The result of testing sequence showed that it shared 98% homology in nucleotide sequence with tomato sucrose phosphate synthase gene ,whose access number is AB051216. This gene had been registered in GenBank.The access number is AY726439.

About this research paper

What this paper is about

PCR primers were designed based on the consensus domain of some sucrose phosphate synthase genes registered in GenBank. A aimed complete cDNA was obtained by using RT-PCR from tomato fruit . The result of testing sequence showed that it shared 98% homology in nucleotide sequence with tomato sucrose phosphate synthase gene ,whose access number is AB051216. This gene had been registered in GenBank.The access number is AY726439.

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Available abstract

PCR primers were designed based on the consensus domain of some sucrose phosphate synthase genes registered in GenBank. A aimed complete cDNA was obtained by using RT-PCR from tomato fruit . The result of testing sequence showed that it shared 98% homology in nucleotide sequence with tomato sucrose phosphate synthase gene ,whose access number is AB051216. This gene had been registered in GenBank.The access number is AY726439.

Key concepts: GenBank, Sucrose-phosphate synthase, Gene, Biology, Complementary DNA, Homology (biology), Cloning (programming), Genetics

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