Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS

Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOFMS).Methods A ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8 μm) column was used to separate.The mobile phase consisted of water containing 0.1 % methane acid and acetonitrile was used as gradient elute.The flow rate was 0.4 ml/min.TOFMS was applied for qualitative analysis under positive ion mode.Results Under optimized condition,19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions.Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata.