2013•PubMedRequires access

[Analysis on chemical constituents of Gardenia jasminoides by UHPLC-Q-TOFMS].

Xiaoyan Wang, Zhang Li, Tianqi Wang, Zhenyu Zhu

Abstract

OBJECTIVE: To analyze the chemical constituents of Gardenia jasminoides by UHPLC-Q-TOFMS. METHODS: A ACQUITY UPLC HSS T3 (100 mm x 2.1 mm, 1.8 microm) column was used. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used in gradient elution. The flow rate was 0.4 mL/min. TOFMS was applied for qualitative analysis under negative ion mode. RESULTS: Under optimized condition, 18 major constituents in Gardenia jasminoides were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. CONCLUSION: A simple and reliable method using UHPLC-Q-TOFMS is established and can be used to identify the chemical constituents of Gardenia jasminoides.

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Key concepts: Gardenia jasminoides, Chromatography, Chemistry, Mass spectrometry, Gardenia, Chemical constituents, Gradient elution, Analytical Chemistry (journal)