Isolation,Identification and Degradation Characteristics of a Phenol-degrading Rhodococcus sp.

A strain(11-111) isolated from the plant rhizospheere soil nearby Tangshan Wastewater Treatment Plant was able to grow on phenol as sole carbon and energy source.The highest endurance capability of the strain for phenol was 2,000 mg/L.The factors affecting phenol degrading were investigated,and it showed better phenol degrading rate at pH 6.5~8.5,24~32 ℃,liquid volume no more than 1/5,160 r/min of shaking rate,initial concentration of 500~1 500 mg/L phenol as the substrate.It was identified as a Rhodococcus sp.,based on 16S rRNA gene sequences,biochemical and physiological characteristics.The strain has research value and application prospects because of a strong ability to adapt to environment and phenol degradation.