Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction

Objective:To study the relationship between the single nucleotide polymorphisms (SNP)936C/T,-460T/C,405G/C in Vascular endothelial growth factor (VEGF)gene and their association with cerebral infarction (CI).Methods:Case-control study was carried out to investigate allelic genotypes and frequency of the three VEGF SNP in 150 subjects with CI and 120 control individuals.Polymerase chain reaction-Restriction fragment lengthpolymorphism,(PCR-RFLP)[U3] and DNA sequencing were used to analyze the polymorphisms.The correlations between VEGF SNPs and CI were evaluated.Results:There was a highly significant difference in allele frequency of 936C/T SNP between CI and control subjects,and the T allele frequencies were respectively 0.3667 versus 0.2500(P0.01).The frequencies of 936C/T SNP genotypes were significantly different(P=0.001)between the CI group(CC:34.00%,CT:58.67%,TT:7.33%)and the control group (CC:55.83%,CT:38.33%,TT:5.83%).The frequency (66.00%)of genotype (CT+TT)of 936C/T SNP in CI group were significantly higher than that (44.17%)in the control group (P=0.001).The frequencies of allele T and genotype (CT+TT)of VEGF936C/T SNP were still associated with CI after stratification of blood pressure.No difference was found in the genotype and allelic frequencies of VEGF-460T/C,405G/C alleles between CI group and the control groups.Conclusion:Our results demonstrated that VEGF936C/T SNP might be associated with CI and be an independent risk factor of CI.But we did not find that VEGF-460T/C,405G/C SNP associated with CI.