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Cloning and Expression of a Human Neuritin cDNA

Yi Xie

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Abstract

A human cDNA of 1 618 bp was cloned from human fetal brain cDNA library. The cDNA fragment con- tained an ORF encoding a 142-aa protein with a calculated molecular mass of 15.3 ku. Through the current data base search and comparison, the cDNA was found to have 98 % of homology with rat new-itin gene. Multiple tissue North-ern blot analysis showed that the neuritin was specifically expressed in brain. The proper protein was expressed and purified by constructing the ORF fragment into the pQE4O expression vector.

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What this paper is about

A human cDNA of 1 618 bp was cloned from human fetal brain cDNA library. The cDNA fragment con- tained an ORF encoding a 142-aa protein with a calculated molecular mass of 15.3 ku. Through the current data base search and comparison, the cDNA was found to have 98 % of homology with rat new-itin gene. Multiple tissue North-ern blot analysis showed that the neuritin was specifically expressed in brain. The proper protein was expressed and purified by constructing the ORF fragment into the pQE4O expression vector.

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Available abstract

A human cDNA of 1 618 bp was cloned from human fetal brain cDNA library. The cDNA fragment con- tained an ORF encoding a 142-aa protein with a calculated molecular mass of 15.3 ku. Through the current data base search and comparison, the cDNA was found to have 98 % of homology with rat new-itin gene. Multiple tissue North-ern blot analysis showed that the neuritin was specifically expressed in brain. The proper protein was expressed and purified by constructing the ORF fragment into the pQE4O expression vector.

Key concepts: Complementary DNA, cDNA library, Molecular biology, Cloning (programming), Homology (biology), Biology, Molecular cloning, Northern blot

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