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Cloning of a Human Arsenic Resistance Related Gene (hARRG) cDNA and Its Expression in E.coli

Pan Ze

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Abstract

A novel human full length cDNA was cloned from a fetal brain cDNA library through a high throughput cDNA sequencing project. It showed 88 % identity in nucleic acid sequence to asr 2 of hamster. It was named human arsenite resistance related gene ( hARRG ). Moverover hARRG was almost completely identity to ARS 2 (GenBank No. AF082871) except that hARRG was 253 bp shorter than ARS 2. It seems not a full length gene. A full length hARRG cDNA of 2 999 bp was cloned with Northern blot, electron RACE and 5′ SMART RACE. This cDNA is located in chromosome 7q21.3 by Unigene. It has a integrated ORF, encoding a protein of 788 amino acids, with putative molecular weight of 89.2 ku. The ORF of hARRG was subcloned into reconstructed PET11c plasmid and found expressed in E. coli . with IPTG inducing.

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What this paper is about

A novel human full length cDNA was cloned from a fetal brain cDNA library through a high throughput cDNA sequencing project. It showed 88 % identity in nucleic acid sequence to asr 2 of hamster. It was named human arsenite resistance related gene ( hARRG ). Moverover hARRG was almost completely identity to ARS 2 (GenBank No. AF082871) except that hARRG was 253 bp shorter than ARS 2. It seems not a full length gene. A full length hARRG cDNA of 2 999 bp was cloned with Northern blot, electron RACE and 5′ SMART RACE. This cDNA is located in chromosome 7q21.3 by Unigene. It has a integrated ORF, encoding a protein of 788 amino acids, with putative molecular weight of 89.2 ku. The ORF of hARRG was subcloned into reconstructed PET11c plasmid and found expressed in E. coli . with IPTG inducing.

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Available abstract

A novel human full length cDNA was cloned from a fetal brain cDNA library through a high throughput cDNA sequencing project. It showed 88 % identity in nucleic acid sequence to asr 2 of hamster. It was named human arsenite resistance related gene ( hARRG ). Moverover hARRG was almost completely identity to ARS 2 (GenBank No. AF082871) except that hARRG was 253 bp shorter than ARS 2. It seems not a full length gene. A full length hARRG cDNA of 2 999 bp was cloned with Northern blot, electron RACE and 5′ SMART RACE. This cDNA is located in chromosome 7q21.3 by Unigene. It has a integrated ORF, encoding a protein of 788 amino acids, with putative molecular weight of 89.2 ku. The ORF of hARRG was subcloned into reconstructed PET11c plasmid and found expressed in E. coli . with IPTG inducing.

Key concepts: Complementary DNA, Biology, Molecular biology, Rapid amplification of cDNA ends, cDNA library, Gene, UniGene, Cloning (programming)

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