Treatment of Acute Lung Injury by Anti-inflammatory Response

Objective To investigate the treatment effect of anti-inflammatory response on the expressions of IL-6、IL-10、TNF-α、NF-κΒmRNA in the lung and the levels of IL-6、TNF-α in serum and BALF in rats with lipopolysaccharide-induced acute lung injury(ALI).Methods A total of 48 Spradue Dawley rats were divided into 4 groups randomly:normal saline control group(N group),lipopolysaccharid group(LPS group),N-acetylcysteine group(NAC group),NAC associate with dexamethasone group(NAC+Dex group).The levels of TNF-α、IL-6 were determined by radioimmunoassay.The expresions of IL-6、IL-10、TNF-α、NF-κΒmRNA were detected by RT-PCR,NF-κΒ were determined Western-Blotting.Results Compared with those in the nomml saline control group,the expression levels of IL-6、IL-10、TNF-α、NF-κΒmRNA and NF-κΒ in the injured lungs and the levels of IL-6、TNF-α in serum and BALF increased significantly(P0.01);Compared with those in the LPS group,NAC group and NAC+Dex group decreased significantly(P0.05).Conclusion N-acetylcysteine and dexamethasone can inhibit the production of these inflammatory cytokines in vivo and the mRNA expression in the lung tissues.Anti-inflammatory response may prevente acute lung injury.