2014•Guiding Journal of Traditional Chinese Medicine and PharmacyRequires access

Determination of Oleanolic acid-28-O-β-D-glucopyranoside in Achyranthes Aspera Linnaeus by RP-HPLC

Huang Xiao-ya

Abstract

Objective:To establish a RP-HPLC method for the determinatiom of oleanolic acid-28-O-β-D-glucopyranoside in Achyranthes aspera Linnaeus. Methods:Hibar C18(250 mm×4.6 mm, 5 μm) column was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid(34∶66). The flow rate was 1.0 mL·min-1and the detection wavelength was 203 nm. The column temperature was 35 ℃. Results:Oleanolic acid-28-O-β-D-glucopyranoside showed excellent regression(r=0.9999) at 0.1426-2.851 μg. The average recovery was 102.4%. RSD was 1.7%(n=6). Conclusion:The method is simple, accurate and reproducible, which can be used for determination of oleanolic acid-28-O-β-D-glucopyranoside in Achyranthes aspera Linnaeus.

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Key concepts: Achyranthes aspera, Oleanolic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Medicine, Organic chemistry