2010•Journal of Planar Chromatography – Modern TLCRequires access

Densitometric HPTLC method for analysis of oleanolic acid inAchyranthes asperaL.

Falgun Mehta, Bharat G. Patel, Saurabh Pandya, Keyur B. Ahir

Abstract

Adensitometric HPTLC method has been established for analysis in alcohol extracts of the seeds and leaves of Achyranthes aspera L. The extracts were separated on aluminum foil-backed silica gel 60 F 254 plates. Detection was by measurement of absorbance at 490 nm, in absorbance/reflectance mode, after derivatization with 10% H 2 SO 4 in 95.5% ( v/v ) ethanol. The RF of oleanolic acid was 0.22. The oleanolic acid content of both extracts was compared statistically and the amount in the seed extract found to be higher (0.970%) than that in the leaf extract (0.666%).

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Key concepts: Achyranthes aspera, Oleanolic acid, Derivatization, Absorbance, Chromatography, Chemistry, Ethanol, Silica gel