Culture conditions optimization of rotavirus LH9 on Vero cells

In order to optimize the culture conditions of rotavirus strains on Vero cells,the strain LH9 was inoculated in cell culture bottles with different volume(100ml,2000ml,3L,15L)by 0.1 MOI.The two inoculation methods(adsorption or non-adsorpttion) and two harvesting methods(direct centrifugalizing and centrifugalizing after freeze-thaw) were adopted to observe the effects of these methods on the virus titers.The viability of the cell was analyzed using the CASY Cell Counter,the CPE of Vero cells after inoculation was observed day by day and the virus titers were determined by CCID50 test.The results showed that the virus titers are higher when using adsorption method and freeze-thaw technique.The virus titer is the highest(6.0 - 7.0 logCCID50/ml).in the case of 15L cell culture bottles.