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Cloning and sequence analysis of Jilin isolated 18S rRNA gene of Theileria sp

Zhang Shou-fa

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Abstract

To analyse the 18S rRNA nucleotide sequence of Theileria sp in Jilin,a pair of primers were designed according to the 18S ribosomal RNA(18S rRNA) gene sequences pulished in the GenBank.The 18S rRNA gene of the Jilin isolate was amplified by PCR and cloned into pMD18-T vector.The recombinant plasmid were transformed into E.coli DH5α and identified by HindⅢ digestion and PCR.The fragment was sequenced and the sequence of nucleotides was analysed with published Theileria sp 18S rRNA gene sequence.Results showed that the fragment size is 459 bp and the homology of nucleotide sequence between Jilin isolate and China 1 strain was 100%.

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What this paper is about

To analyse the 18S rRNA nucleotide sequence of Theileria sp in Jilin,a pair of primers were designed according to the 18S ribosomal RNA(18S rRNA) gene sequences pulished in the GenBank.The 18S rRNA gene of the Jilin isolate was amplified by PCR and cloned into pMD18-T vector.The recombinant plasmid were transformed into E.coli DH5α and identified by HindⅢ digestion and PCR.The fragment was sequenced and the sequence of nucleotides was analysed with published Theileria sp 18S rRNA gene sequence.Results showed that the fragment size is 459 bp and the homology of nucleotide sequence between Jilin isolate and China 1 strain was 100%.

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Available abstract

To analyse the 18S rRNA nucleotide sequence of Theileria sp in Jilin,a pair of primers were designed according to the 18S ribosomal RNA(18S rRNA) gene sequences pulished in the GenBank.The 18S rRNA gene of the Jilin isolate was amplified by PCR and cloned into pMD18-T vector.The recombinant plasmid were transformed into E.coli DH5α and identified by HindⅢ digestion and PCR.The fragment was sequenced and the sequence of nucleotides was analysed with published Theileria sp 18S rRNA gene sequence.Results showed that the fragment size is 459 bp and the homology of nucleotide sequence between Jilin isolate and China 1 strain was 100%.

Key concepts: 18S ribosomal RNA, Biology, GenBank, Homology (biology), Gene, Sequence analysis, Ribosomal RNA, Nucleic acid sequence

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