2002•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Content of Cinnamaldehyde and Cinnamic Acid in Xiaoliu Capsules by RP-HPLC

Yang Song

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Abstract

Objective: To determine cinnamaldehyde and cinnamic acid in Xiaoliu Capsules. Methods: RP HPLC method was used. The samples were separated on a Luna C 18 column with the mobile phase of acetonitrile 0.1% phosphoric acid (32∶78) and the detection wavelength was set at 285 nm.Results: The calibration curve were linear in the range of 2.63~26.3 μg/ml cinnamaldehyde ( r =0.999 6) and 0.736~7.36 μg/ml cinnamic acid ( r =0.999 9). Its average recovery were 98.4 % and 98.7%. Conclusion: The method is quick, accurate and with a good reproducibility and can be used to control the quality of the preparation.

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What this paper is about

Objective: To determine cinnamaldehyde and cinnamic acid in Xiaoliu Capsules. Methods: RP HPLC method was used. The samples were separated on a Luna C 18 column with the mobile phase of acetonitrile 0.1% phosphoric acid (32∶78) and the detection wavelength was set at 285 nm.Results: The calibration curve were linear in the range of 2.63~26.3 μg/ml cinnamaldehyde ( r =0.999 6) and 0.736~7.36 μg/ml cinnamic acid ( r =0.999 9). Its average recovery were 98.4 % and 98.7%. Conclusion: The method is quick, accurate and with a good reproducibility and can be used to control the quality of the preparation.

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Available abstract

Objective: To determine cinnamaldehyde and cinnamic acid in Xiaoliu Capsules. Methods: RP HPLC method was used. The samples were separated on a Luna C 18 column with the mobile phase of acetonitrile 0.1% phosphoric acid (32∶78) and the detection wavelength was set at 285 nm.Results: The calibration curve were linear in the range of 2.63~26.3 μg/ml cinnamaldehyde ( r =0.999 6) and 0.736~7.36 μg/ml cinnamic acid ( r =0.999 9). Its average recovery were 98.4 % and 98.7%. Conclusion: The method is quick, accurate and with a good reproducibility and can be used to control the quality of the preparation.

Key concepts: Cinnamaldehyde, Cinnamic acid, Chromatography, Phosphoric acid, Calibration curve, High-performance liquid chromatography, Chemistry, Content determination

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