Gene transfection of human NT3-cDNA into the NIH3T3 fibroblasts

AIM: To establish a NIH3T3 cell line which expresses human NT3 and to observe the growth of cochlear ganglion cells by co culturing. METHODS: The NT3 cDNA gene was transfected into NIH3T3 cell line by using cationic liposome (LP2000). The NIH3T3 cell transfected with NT3 cDNA was examined by RT PCR, Western blot and immunoreactivity methods. The cochlear ganglion cells were co culturing with the NIH3T3 cells transfered with NT3. RESULTS : Cell clones with G 418 resistance were obtained, the size of RT PCR product of the positive cell clones being about 744 bp, and the positive signals of protein were obtained. The number of cochlear ganglion cells did not reduce after co culturing with NIH3T3 cells transfected with NT3. CONCLUSION: The human NT3 cDNA has been successfully transfected into the NIH3T3 cells and these cells benefit the growth of cochlear ganglion cells.