Inhibition of the expression of proliferation cell nuclear antigen in osteosarcoma cells by a short hairpin RNA

OBJECTIVE:To construct an expression plasmid of a short hairpin RNA target proliferation cell nuclear antigen and investigate its inhibitory effect on the expression of PCNA in osteosarcoma cell MG-63 in vitro. METHODS: An expression plasmid of a short hairpin RNA target PCNA was constructed and transfected to MG-63 cell by liposome. The expressions of PCNA mRNA and protein were examined by RT-PCR and the immunohistochemical method, respectively. The inhibition of the cell proliferation was estimated by MTT method and the clonal forming test. RESULTS: The number of the clone after transfection was significantly smaller than the other groups. PCNA shRNA significantly reduced the expressions of PCNA mRNA and protein. The expressions of PCNA mRNA at 24, 48, 72 h after transfection were inhibited by 68.62%, 80.62% and 73.12%, respectively, and the PI values of the transfected group at 24, 48 and 72 h after transfection were 47.72%, 23.37% and 35.90% of the control group. PCNA shRNA also inhibited the growth of the cell by MTT method. The inhibition rate reached 68.89% at 48 h after transfection. CONCLUSION: PCNA shRNA can significantly reduce the expressions of PCNA mRNA and protein and inhibit the proliferation of the MG-63 cell.