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Application of RAPD in Fangfeng

Jingpeng Li

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Abstract

To define the molecular characters of differentiating Fangfeng Saposhnikovia divaricata (Turcz.) Schischk, from their allied species Friocycla albescens(Franch), P. dielsianum Fedde ex wolff, Peucedanum ledebourieuoides K, T, Fu, Curum Carvil by RAPD technique. General DNA was isolated from fresh Fangfeng and their allied species by SDS method, The random amplified Polymorphic DNA(RAPD) was used to identify them. 8 effective primers were selected from 21 primers. Cluster analysis tree were constructed by using genetic distance UPGMA method. The RAPD results suit for the ananlysis of Rodix Gentianae species in genetic polymorphism and genetic relationship, The method of RAPD can be used to identify the Fangfeng species and its allied species.

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What this paper is about

To define the molecular characters of differentiating Fangfeng Saposhnikovia divaricata (Turcz.) Schischk, from their allied species Friocycla albescens(Franch), P. dielsianum Fedde ex wolff, Peucedanum ledebourieuoides K, T, Fu, Curum Carvil by RAPD technique. General DNA was isolated from fresh Fangfeng and their allied species by SDS method, The random amplified Polymorphic DNA(RAPD) was used to identify them. 8 effective primers were selected from 21 primers. Cluster analysis tree were constructed by using genetic distance UPGMA method. The RAPD results suit for the ananlysis of Rodix Gentianae species in genetic polymorphism and genetic relationship, The method of RAPD can be used to identify the Fangfeng species and its allied species.

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Available abstract

To define the molecular characters of differentiating Fangfeng Saposhnikovia divaricata (Turcz.) Schischk, from their allied species Friocycla albescens(Franch), P. dielsianum Fedde ex wolff, Peucedanum ledebourieuoides K, T, Fu, Curum Carvil by RAPD technique. General DNA was isolated from fresh Fangfeng and their allied species by SDS method, The random amplified Polymorphic DNA(RAPD) was used to identify them. 8 effective primers were selected from 21 primers. Cluster analysis tree were constructed by using genetic distance UPGMA method. The RAPD results suit for the ananlysis of Rodix Gentianae species in genetic polymorphism and genetic relationship, The method of RAPD can be used to identify the Fangfeng species and its allied species.

Key concepts: RAPD, UPGMA, Biology, Botany, Genetic distance, Veterinary medicine, Genetics, Genetic diversity

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Application of RAPD in Fangfeng — Research Paper | ScholarLens