ScholarLens Back to search
2004•Xi'nan Nongye Daxue xuebaoRequires access

RAPD-BASED MOLECULAR IDENTIFICATION OF DENDROBIUM SPECIES

Rui Peng

Open publisher page 1 citations

Abstract

Fifteen species of the genus Dendrobium were analyzed, using RAPD (random amplified polymorphic DNA) technique. Ten decanucleotide primers (10 bp) were selected from 70 primers, and 99 bands of amplification were obtained, which were used for RAPD analysis. Based on the RAPD profile, a dendrogram of cluster analysis of the species studied was constructed according to their similarity coefficients. It is concluded that RAPD is effective in characterizing Dendrobium species. The high percentage of polymorphic loci ((84.85%)) in the species indicates that this genus is highly diverse genetically.

About this research paper

What this paper is about

Fifteen species of the genus Dendrobium were analyzed, using RAPD (random amplified polymorphic DNA) technique. Ten decanucleotide primers (10 bp) were selected from 70 primers, and 99 bands of amplification were obtained, which were used for RAPD analysis. Based on the RAPD profile, a dendrogram of cluster analysis of the species studied was constructed according to their similarity coefficients. It is concluded that RAPD is effective in characterizing Dendrobium species. The high percentage of polymorphic loci ((84.85%)) in the species indicates that this genus is highly diverse genetically.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Fifteen species of the genus Dendrobium were analyzed, using RAPD (random amplified polymorphic DNA) technique. Ten decanucleotide primers (10 bp) were selected from 70 primers, and 99 bands of amplification were obtained, which were used for RAPD analysis. Based on the RAPD profile, a dendrogram of cluster analysis of the species studied was constructed according to their similarity coefficients. It is concluded that RAPD is effective in characterizing Dendrobium species. The high percentage of polymorphic loci ((84.85%)) in the species indicates that this genus is highly diverse genetically.

Key concepts: RAPD, Dendrobium, Dendrogram, Biology, Genus, Similarity (geometry), Botany, Genetic diversity

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RAPD-BASED MOLECULAR IDENTIFICATION OF DENDROBIUM SPECIES — Research Paper | ScholarLens