A New Method for Monitoring the Genetic Quality of BALB/c Mice

Objective In order to explore a new method for monitoring the genetic quality of laboratory animals,a comparison of RAPD map and STR map with the biochemical labeling in monitoring inbred BABL/c mouse was carried out.Methods DNA was extracted from the tail of inbred BALB/c mice.6 pairs of RAPD primers and 20 pairs of STR primers were used to amplify the mouse DNA.13 loci of biochemical analysis were used to monitor the quality of inbred BALB/c mouse.Results The amplified band patterns were obviously different in the products of 4 RAPD primers(p2,p3,p5 and p6) and 4 STR primers(2,4,10,11),and 6 loci depicted by biochemistry were different in the inbred BALB/c mice.Conclusion The results indicate that to a certain extent the results of RAPD and STR are comparable with that of biochemical labeling,and RAPD and STR can be used to monitoring the genetic quality of inbred laboratory animals.