Expression and clinical significance of TGF-β1 and VEGF in colon carcinoma tissue

Objective: To explore the expression,relationship and clinical significance of TGF-β1 and VEGF in carcinoma tissues of patients with colon carcinoma.Methods: The protein expression of TGF-β1 and VEGF was detected in specimens from 65 cases of colon carcinoma and 39 cases of normal colon mucosa tissues with immunohistochemical method,and the mRNA expression was detected with reverse transcription(RT)-PCR.Results: Our results showed that the positive rates of TGF-β1 and VEGF protein detected with immunohistochemical method in colon carcinoma group were significantly higher than those in the normal control group(P0.05),and the mRNA expression of TGF-β1 and VEGF in colon carcinoma group were both significantly higher than those in the normal control group.Patients in T 3 ～T 4,Dukes C、D and with lymph node metastasis had much higher expression rates of TGF-β1 and VEGF than patients in T 1 ～T 2,Dukes A、B and without lymph node metastasis(P0.05).Significant correlation was also found between TGF-β1 and VEGF expressions(P0.05).Conclusion: TGF-β1 and VEGF are up-regulated in colon carcinoma,and they are significantly associated with the depth of invasion,the lymph node metastasis and the stage of disease,and there is significant correlation between TGF-β1 and VEGF expressions.TGF-β1 and VEGF might be associated with tumor progression and disease prognosis,and may provide reference for prognostic evaluation of colon carcinoma.