FAC inhibits proliferation,induces apoptosis of human breast cancer MCF-7,increases expression of p53 and decrease expression of c-Myc and NF-κB

Objective:To research the effects of flavonoids of Astragalus complanatus(FAC) on proliferation,apoptosis and expressions of p53,c-My and NF-кB in human breast cancer MCF-7 cells.Methods:The inhibit rate of MCF-7 cells treated by different concentration of FAC was studied by MTT at different times.The apoptosis rate of MCF-7 cell was essayed by flow cytometry after treated with effective concentration of FAC and time points according to MTT.The expressions of p53,c-Myc and NF-кB were quantified by fluorescence immunoassay and Western blot.Results:According to MTT,0.0625,0.125,0,25,0.5 mg/ml of FAC treated for 24 h,48 h,72 h markedly inhibited proliferation of MCF-7 (P 0.05,vs control).According to flow cytometry,MCF-7 was induced to apoptosis by FAC.After 72 h treatment with FAC(0.0625,0.125,0.25,0.5 mg /ml),the apoptosis rates were 6.5%,18.3%,49.6% and 57.9%,respectively.The expression of p53 was up-regulated,while c-Myc and NF-κB were markedly reduced detected by fluorescence immunoassay and Western blot.Conclusion:FAC could inhibit proliferation and induce apoptosis of MCF-7.Its role may be related to decreased expression of NF-кB and c-Myc,increased expression of p53.